Pooled in Vivo Experiments
Mosaic Screening is Gordian’s unique version of pooled in vivo screening in which thousands of targets and therapies are tested in a single sick animal.
Unlike typical in vivo screening, Mosaic Screening uses animals more representative of human patients with the same disease, creating a complicated biological environment similar to what the therapies will encounter in clinical trials.
A pooled library of gene therapies is introduced into the target tissue, turning it into a “mosaic.” Various cells receive different individual therapies, each with a unique barcode, while the rest of the tissue remains unperturbed.
We single-cell sequence the treated tissue and use the library’s unique barcodes to identify the specific treatment given to each cell. The single-cell transcriptome allows us to explore various aspects of disease biology simultaneously in an environment very similar to where a clinical drug would need to be effective.
Patient Avatars are animals that are most closely representative of human patients with age-related diseases, and have often acquired the disease naturally.
Instead of focusing on just one hypothesis about a specific disease, Gordian’s Pythia examines the entire transcriptome (the complete set of RNA molecules expressed from the Patient Avatar’s genes) to see how potential therapies affect various cellular pathways.